597 papers
Proteomic analysis of KNL1 in HEK 293T/17 cells treated with microtubule-disrupting agents reveals 111 phosphorylation sites, demonstrating that this outer kinetochore protein is extensively phosphoregulated in response to defective kinetochore-microtubule attachments.
This paper presents an optimized full spectrum flow cytometry protocol and control strategy that overcomes complex tissue autofluorescence to enable high-dimensional proteomic phenotyping and isolation of rare endothelial cell subpopulations from solid murine tissues.
This study reveals that the nematode-trapping fungus *Arthrobotrys oligospora* integrates nematode-induced NADPH oxidase signaling with cell polarity cues and cytoskeletal reorganization to orchestrate the morphological transition required for trap formation.
This study presents a comprehensive multi-omics atlas of the murine glycoproteome across 19 tissues, revealing pronounced sex-specific differences in glycosylation patterns driven by coordinated regulation of protein abundance and glyco-enzyme expression, particularly in the liver, kidney, and salivary gland, while the brain glycome remains largely conserved.
This paper presents a stochastic phase field model demonstrating that cells can effectively sense immobilized ECM gradients (haptotaxis) and maintain robust directional migration even when faced with competing cues or ligand removal, a process where increased directional persistence compensates for reduced bias at the cost of greater cell-to-cell heterogeneity.
This study reveals that culturing human umbilical cord mesenchymal stem cells (hUC-MSCs) under hypoxia induces metabolic reprogramming and a partial cancer stem cell-like phenotype with accelerated proliferation, raising significant safety concerns regarding their clinical application despite their enhanced therapeutic potential.
This study demonstrates that sex-specific differences in osteoblast extracellular matrix maturation, rather than soluble factors like VEGF, drive divergent, contact-dependent regulation of endothelial cell behavior, thereby contributing to skeletal vascular dimorphism.
This study establishes a robust, quantitative in vitro platform for drug screening by demonstrating that thermally activated calcium sulfate induces reproducible, dose-dependent vacuolation in mammalian cells, which can be effectively monitored via Neutral Red uptake to distinguish between specific vacuole inhibitors and cytotoxic compounds.
This study demonstrates that Dynamin-2, in conjunction with Endophilin-B1, mediates the fission-mediated retrieval of Atg9A from phagophores to prevent its degradation and ensure efficient autophagosome formation.
This study characterizes novel KIF22 variants in human chondrocytes, revealing that heterozygous mutations cause dominant mitotic defects through constitutive activation while the recessive R49Q variant leads to milder, partially penetrant segregation errors via mixed-state dysregulation, thereby expanding the known genotypic and mechanistic landscape of SEMDJL2.
This study identifies and characterizes two distinct, pro-fibrotic endothelial-to-mesenchymal transition-derived subpopulations, specifically TAGLN+ cells, which emerge early in metabolic dysfunction-associated steatotic liver disease and correlate with poor patient outcomes, offering new targets for diagnostics and therapeutics.
This study establishes a two-dimensional in vitro model using celiac disease patient-derived iPSC small intestinal epithelial cells, revealing that while they mature similarly to healthy controls, they exhibit inherent differences in inflammation-related gene expression and display a more persistent, attenuated response to cytokine stimuli.
This paper demonstrates that performing a controlled sublimation step within the SEM chamber prior to FIB milling effectively mitigates surface ice and curtaining artifacts in cryo-ET lamella preparation without compromising sample vitrification or structural integrity.
This study demonstrates that canonical autophagy is dispensable for the endosomal-exosomal trafficking of plasma membrane PD-L1, whereas the actin-capping protein CAPZ serves as a critical regulator of this pathway by controlling endosomal maturation and sorting decisions that determine PD-L1 abundance on the cell surface.
Using patient-specific induced pluripotent stem cell-derived smooth muscle cells, this study reveals that sotatercept treats pulmonary arterial hypertension not only by blocking smooth muscle cell-to-myofibroblast transition but also by rapidly disrupting a pathological TGF-beta/collagen-integrin feedback loop, thereby uncovering new mechanisms for its variable and rapid clinical efficacy.
This study demonstrates that in human macrophages, the actin-binding protein drebrin functions as a multivalent cortical hub by linking microtubule plus-ends via EB3 and recruiting clathrin-mediated endocytosis, thereby facilitating the specific endocytic transport of β2 integrin at podosomes.
This study reveals that extracellular protein aggregation impairs endosomal recycling of signaling receptors, leading to reduced phosphorylation and degradation of the repressor ZIP-3, which subsequently activates the transcription factor ATFS-1 to restore extracellular proteostasis and promote organismal resilience.
This study reveals that immune-activated lipid droplets serve as polyunsaturated fatty acid reservoirs, particularly arachidonic acid, which are mobilized to drive bacterial clearance and inflammation through prostaglandin synthesis and enhanced phagocytosis.
This study reveals that c-MYC is an aggregation-prone, amyloidogenic protein whose spontaneous formation of amyloid oligomers induces transcription-independent apoptosis, suggesting a built-in failsafe mechanism that contributes to its paradoxical tumor-suppressing activity.
Using live-imaging of over 150 HCT116 cell lineages, this study reveals that multipolar chromosome segregation in early mitosis is the primary factor limiting post-whole-genome duplication proliferation, with successful lineages either mitigating this risk in one sub-lineage or surviving despite early multipolar events.